Journal: bioRxiv

Article Title: Serum Amyloid P inhibits single stranded RNA-induced lung inflammation, lung damage, and cytokine storm in mice

doi: 10.1101/2020.08.26.269183

Figure Lengend Snippet: (A) The total number of cells collected from the BALF at day 3 post ORN06 aspiration. Cell spots of BALF at day 3 post ORN06 aspiration were stained with Wright-Giemsa stain and the percentage of (B) monocytes/macrophages, (C) lymphocytes, and (D) neutrophils was determined by examining at least 150 cells per mouse BAL sample. Values are mean ± SEM, n = 6 mice (3 males and 3 females), except control group where n = 8 (3 males and 5 females). ** p < 0.01 and **** p < 0.0001 (1-way ANOVA, Bonferroni’s test). For A-D, there was no significant difference between the control group and the SAP-treated group.

Article Snippet: After air drying for 48 hours at room temperature, some of the prepared BALF cell spots were fixed and treated with Wright-Giemsa stain (Polysciences, Inc., Warrington, PA) following the manufacturer’s instructions.

Techniques: Staining, Giemsa Stain

Journal: bioRxiv

Article Title: Serum Amyloid P inhibits single stranded RNA-induced lung inflammation, lung damage, and cytokine storm in mice

doi: 10.1101/2020.08.26.269183

Figure Lengend Snippet: Cell spots of BALF at day 3 were stained with Wright-Giemsa stain. The left two images are of BALF cell spots from two different control male mice. The middle two images are of BALF cell spots from two different male mice treated with buffer after ORN06 insult. The right two images are of BALF cell spots from two different male mice treated with SAP after ORN06 insult. Bar is 10 µm.

Article Snippet: After air drying for 48 hours at room temperature, some of the prepared BALF cell spots were fixed and treated with Wright-Giemsa stain (Polysciences, Inc., Warrington, PA) following the manufacturer’s instructions.

Techniques: Staining, Giemsa Stain